FastQCFastQC Report
Wed 15 Apr 2020
FCHHWFYBBXX_L3_CHKPEI85217070035_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHHWFYBBXX_L3_CHKPEI85217070035_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81119211
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG5778230.7123133877621172No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAC4014160.49484702211908843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG149550.015.51381743
CGAGCCC279500.012.83559242
CCGAGCC343600.012.22917143
TATACTG726100.011.6747785
TCCGAGC366200.011.57417542
CTGTTAA548300.011.5701111
GTATTAT229700.011.5427591
TCTATAC138750.011.4418993
CTATACT651500.011.4222454
TAACAGT794000.011.4204414
GTATAGG474300.011.2937651
GTATTAG150250.011.2190941
GTATTAA223500.011.1781871
GTGTAGG243550.010.8775031
CCTATAC577900.010.8435233
GTATAGA148750.010.8395211
GAGCCCA334350.010.82629643
TATAGGC435950.010.759812
ATAGGCC449350.010.7520513
TAATACT187850.010.7384694
Produced by FastQC (version 0.11.5)